One-Step Multiplex PCR Strategy for Identification of Mutations by SSCP and DNA Sequencing
نویسندگان
چکیده
منابع مشابه
Improved detection and characterization of mutations by primer addition in nonradioisotopic SSCP and direct PCR sequencing.
Single-strand conformation polymorphism (SSCP) has become the most widely used method to screen new mutations or already-established polymorphisms. Several protocols have been described to overcome the two main problems related to this technique, which are separation of both strands and detection. For the former, there are methods using a strong denaturant such as methylmercury hydroxide to enh...
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By simultaneously amplifying more than one locus in the same reaction, multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory. While numerous papers and manuals discuss in detail conditions influencing the quality of PCR in general, relatively little has been published about the important experimental factors and the common difficulties...
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Single-strand conformation polymorphism (SSCP) is a powerful technique used to identify mutations in short pieces of DNA (1). In this technique, the DNA of interest is often amplified by the polymerase chain reaction (PCR) and then denatured by heat or alkali treatment before electrophoresis on a non-denaturing polyacrylamide gel. Differences in mobility of either of the single strands, compare...
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T e c h n i c a l progress in the determination of DNA sequence has resulted in an enormous amount of DNA sequence data, with technical innovations such as the polymerase chain reaction (PCR) accelerating the rate of increase of this data. This combination of data and techniques makes it possible now to exploit sequence differences between individuals. Such studies not only can answer biologica...
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30 milled rice samples were collected from retailers in four states of Malaysia. These samples were evaluated for Eurotium spp. contaminations by direct plating on malt extract salt agar (MESA). All Eurotium were isolated and identified based on morphology and nucleotide sequences of internal transcribed spacer 1 (ITS1) and ITS2 of the rDNA. Four Eurotium species (E. rubrum, E. amstelodami, E....
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00292bm06